Diagnostic Power of Cytokine M-CSF, Metalloproteinase 2 (MMP-2) and Tissue Inhibitor-2 (TIMP-2) in Cervical Cancer Patients Based on ROC Analysis.

Abstract

Macrophage colony-stimulating factor (M-CSF), matrix metalloproteinase-2 (MMP-2) and its specific tissue inhibitor (TIMP-2) may play an important role in the pathogenesis of cancer disease. We investigated the plasma levels and diagnostic power (ROC curve analysis) of M-CSF, MMP-2, TIMP-2 and tumor markers CA 125 and SCC-Ag in cervical cancer (CC) patients as compared to control group. The study included 89 patients with cervical cancer. The control group consisted of 50 healthy, untreated women. The plasma levels of M-CSF, MMP-2 and TIMP-2 were determined using ELISA, CA 125 and SCC-Ag - by CMIA method. The median levels of M-CSF, TIMP-2, SCC-Ag and CA 125 in the entire group of CC were significantly different than compared to the healthy women group. MMP-2 showed the highest value of sensitivity from all examined parameters (in stage I of CC - 93.10%, II - 82.76%, III and IV - 96.88%, total group - 92.05%). The highest specificity was obtained by M-CSF (86%). The area under the ROC curve (AUC) of M-CSF (0.8051) was the largest of all the tested parameters (even higher than commonly used tumor markers) in the group of cervical cancer. The combination of M-CSF, MMP-2 or TIMP-2 with SCC antigen resulted in an increase AUCs in all cases (0.8760;0.7880;0.8081;respectively). The findings of this study suggest the usefulness of all examined parameters in the diagnostics of CC patients. Out of the tested substances, M-CSF also appears to be the best candidate for cancer diagnostics in all stages of the disease, based on ROC analysis.

• Authors: Emilia Lubowicka, Monika Zbucka-Kretowska, Iwona Sidorkiewicz, Monika Zajkowska, Ewa Gacuta, Andrzej Puchnarewicz, Lech Chrostek, Maciej Szmitkowski, Sławomir Ławicki
• Journal: Pathology oncology research : POR (Pathol Oncol Res) Vol. 26 Issue 2 Pg. 791-800 (Apr 2020) ISSN: 1532-2807 [Electronic] Switzerland
• PMID: 30820752 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• TIMP2 protein, human
• Tissue Inhibitor of Metalloproteinase-2
• Macrophage Colony-Stimulating Factor
• MMP2 protein, human
• Matrix Metalloproteinase 2

Topics

• Adult
• Biomarkers, Tumor (blood)
• Female
• Humans
• Macrophage Colony-Stimulating Factor (blood)
• Matrix Metalloproteinase 2 (blood)
• Middle Aged
• ROC Curve
• Sensitivity and Specificity
• Tissue Inhibitor of Metalloproteinase-2 (blood)
• Uterine Cervical Neoplasms (blood, diagnosis)
• Young Adult