Studies on the major common precipitating antigen of capripoxvirus.

Abstract

The proteins of sheep pox, goat pox, sheep and goat pox and lumpy skin disease (Neethling) viruses were labelled with [35S]methionine. The major structural polypeptides of these viruses co-migrated on polyacrylamide gels, demonstrating the very close biochemical relationship between them. Using the agar gel immunodiffusion (AGID) test with radiolabelled antigen preparations, a major common precipitating antigen was identified. This co-migrated on polyacrylamide gels with one of the major structural polypeptides [mol. wt. 67000 (67K)]. The use of [35S]methionine-labelled antigen preparations considerably improved the sensitivity of the AGID test as a diagnostic test for capripoxvirus antibody detection.

• Authors: R P Kitching, J M Hammond, D N Black
• Journal: The Journal of general virology (J Gen Virol) Vol. 67 ( Pt 1) Pg. 139-48 (Jan 1986) ISSN: 0022-1317 [Print] England
• PMID: 3080547 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Sulfur Radioisotopes
• Viral Proteins
• Methionine

Topics

• Animals
• Antigens, Viral (analysis)
• Cells, Cultured
• Goats
• Immunodiffusion
• Lumpy skin disease virus (analysis)
• Male
• Methionine (metabolism)
• Poxviridae (analysis, immunology, isolation & purification)
• Sheep
• Species Specificity
• Sulfur Radioisotopes
• Testis
• Viral Proteins (analysis)