Enhancement of the effect of BCG vaccine against tuberculosis using DDA/TDB liposomes containing a fusion protein of HspX, PPE44, and EsxV.

Abstract

Tuberculosis has been a major health problem worldwide for years; therefore, it is important to develop and produce an effective vaccine against this disease. In this study, the immunogenicity of Mycobacterium tuberculosis fusion protein (FP) encapsulated in liposomes containing DDA/TDB was evaluated. The FP was expressed in E. coli BL21 and encapsulated in liposomal formulations. Three weeks after the last subcutaneous immunization, IFN-γ, IL-4, IL-17, and IL-12 in spleen cell culture supernatants, and IgG2a, IgG1, and IgG2b titres in sera were measured. The greatest IFN-γ and IL-12 interleukin concentrations were observed in the DDA/TDB/CHOL liposomes containing the FP. Initial injection with BCG improved the efficacy of the DDA/TDB/CHOL/FP vaccine. The IgG2a/IgG1 ratio was also high in the DDA/TDB/CHOL/FP group; furthermore, the IgG2a/IgG1 ratio was increased in the BCG-primed, DDA/TDB/CHOL/FP-boosted group, indicating induction of a cellular immune response. Our study showed that the FP-containing DDA/TDB/CHOL liposomes induced a Th1 response. However, the groups that first received BCG and then DDA/TDB/CHOL/FP had the greatest Th1 response in terms of IFN-γ and IL-12 production of all the groups. This suggests that these formulations enhance the BCG vaccine's effectiveness.

• Authors: Davood Mansury, Kiarash Ghazvini, Saeid Amel Jamehdar, Ali Badiee, Mohsen Tafaghodi, Amin Reza Nikpoor, Yousef Amini, Mahmoud Reza Jaafari
• Journal: Artificial cells, nanomedicine, and biotechnology (Artif Cells Nanomed Biotechnol) Vol. 47 Issue 1 Pg. 370-377 (Dec 2019) ISSN: 2169-141X [Electronic] England
• PMID: 30691310 (Publication Type: Journal Article)

Chemical References

• Antigens, Bacterial
• BCG Vaccine
• Bacterial Proteins
• Cytokines
• ESX-5 protein, Mycobacterium tuberculosis
• Glycolipids
• HspX protein, Mycobacterium tuberculosis
• Liposomes
• PPE44 protein, Mycobacterium tuberculosis
• Quaternary Ammonium Compounds
• Virulence Factors
• trehalose 6,6'-dibehenate
• dimethyldioctadecylammonium

Topics

• Animals
• Antigens, Bacterial (chemistry)
• BCG Vaccine (chemistry, immunology)
• Bacterial Proteins (chemistry)
• Cytokines (metabolism)
• Glycolipids (chemistry)
• Liposomes (chemistry)
• Mice
• Quaternary Ammonium Compounds (chemistry)
• Tuberculosis (metabolism, prevention & control)
• Virulence Factors (chemistry)