Thrombospondin-1 Is a Major Activator of TGF-β Signaling in Recessive Dystrophic Epidermolysis Bullosa Fibroblasts.

Abstract

Mutations in the gene encoding collagen VII cause the devastating blistering disease recessive dystrophic epidermolysis bullosa (RDEB). RDEB is characterized by severe skin fragility and nonhealing wounds aggravated by scarring and fibrosis. We previously showed that TSP1 is increased in RDEB fibroblasts. Because transforming growth factor-β (TGF-β) signaling is also increased in RDEB, and TSP1 is known to activate TGF-β, we investigated the role of TSP1 in TGF-β signaling in RDEB patient cells. Knockdown of TSP1 reduced phosphorylation of smad3 (a downstream target of TGF-β signaling) in RDEB primary fibroblasts, whereas overexpression of collagen VII reduced phosphorylation of smad3. Furthermore, inhibition of TSP1 binding to the LAP/TGF-β complex decreased fibrosis in engineered extracellular matrix formed by RDEB fibroblasts, as evaluated by picrosirius red staining and analyses of birefringent collagen fibrillar deposits. We show that collagen VII binds TSP1, which could potentially limit TSP1-LAP association and subsequent TGF-β activation. Our study suggests a previously unreported mechanism for increased TGF-β signaling in the absence of collagen VII in RDEB patient skin. Moreover, these data identify TSP1 as a possible target for reducing fibrosis in the tumor-promoting dermal microenvironment of RDEB patients.

• Authors: Velina S Atanasova, Rebecca J Russell, Timothy G Webster, Qingqing Cao, Pooja Agarwal, Yok Zuan Lim, Suma Krishnan, Ignacia Fuentes, Christina Guttmann-Gruber, John A McGrath, Julio C Salas-Alanis, Andrzej Fertala, Andrew P South
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 139 Issue 7 Pg. 1497-1505.e5 (07 2019) ISSN: 1523-1747 [Electronic] United States
• PMID: 30684555 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• Collagen Type VII
• SMAD3 protein, human
• Smad3 Protein
• Thrombospondin 1
• Transforming Growth Factor beta

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Cells, Cultured
• Child
• Child, Preschool
• Collagen Type VII (genetics)
• Epidermolysis Bullosa Dystrophica (genetics, metabolism)
• Extracellular Matrix (metabolism)
• Female
• Fibroblasts (metabolism, pathology)
• Fibrosis
• Gene Knockdown Techniques
• Genes, Recessive
• Humans
• Infant
• Infant, Newborn
• Male
• Middle Aged
• Mutation (genetics)
• Phosphorylation
• Protein Binding
• Signal Transduction
• Skin (pathology)
• Smad3 Protein (metabolism)
• Thrombospondin 1 (genetics, metabolism)
• Transforming Growth Factor beta (metabolism)
• Tumor Microenvironment
• Young Adult