Measurement of serum bile alcohol levels in liver dysfunction, using isotope dilution-mass spectrometry.

Abstract

We determined the concentration of two major serum bile alcohols, 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol and 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol, in healthy controls and patients with acute hepatitis, chronic hepatitis, and cirrhosis, using isotope dilution-mass spectrometry. In healthy controls the mean total amount of the two major bile alcohols was 9.6 +/- 3.4 ng/ml (mean +/- SD). In patients with liver diseases the mean values were as follows: acute hepatitis, 44.8 +/- 18.2 ng/ml; chronic hepatitis, 18.2 +/- 5.6 ng/ml; Child grade A cirrhosis, 37.9 +/- 26.0 ng/ml; Child grade B, 42.7 +/- 22.0 ng/ml; and Child grade C, 71.2 +/- 24.2 ng/ml. The mean ratio of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25,26-pentol to 27-nor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol in patients with Child grade C cirrhosis was significantly higher than that in healthy controls (1.42 +/- 0.92 versus 0.61 +/- 0.11; p less than 0.05).

• Authors: T Hiraoka, D Kosaka, G Kajiyama, T Kohda, T Funakura, T Yamauchi, K Kihira, T Hoshita
• Journal: Scandinavian journal of gastroenterology (Scand J Gastroenterol) Vol. 23 Issue 7 Pg. 821-6 (Sep 1988) ISSN: 0036-5521 [Print] England
• PMID: 3067328 (Publication Type: Journal Article)

Chemical References

• Cholestanols

Topics

• Adult
• Aged
• Cholestanols (blood)
• Female
• Gas Chromatography-Mass Spectrometry
• Hepatitis (blood)
• Humans
• Liver Cirrhosis (blood)
• Male
• Middle Aged
• Radioisotope Dilution Technique