Abstract |
Many human bronchial adenocarcinomas have been shown to contain an activated Ki-ras oncogene (Rodenhuis et al., N. Engl. J. Med. 317 929-935, 1987). To test the hypothesis that activated Ki-ras may be causally related to human bronchial carcinogenesis, v-Ki-ras oncogene was transferred into an established human bronchial epithelial cell line, BEAS-2B, by infection with Kirsten murine sarcoma virus (Ki-MSV) or by transfection with a plasmid containing the transforming region of Ki-MSV. These cells formed poorly differentiated adenocarcinomas in athymic nude mice. Cell lines established from these tumors expressed v-Ki-ras p21 protein and were highly tumorigenic. Whereas serum or transforming growth factor beta 1 induced the BEAS-2B cells at clonal density to undergo growth arrest and squamous differentiation, BEAS-2B cells containing activated ras genes were unaffected by transforming growth factor beta 1 and were mitogenically stimulated by serum.
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Authors | R R Reddel, Y Ke, M E Kaighn, L Malan-Shibley, J F Lechner, J S Rhim, C C Harris |
Journal | Oncogene research
(Oncogene Res)
Vol. 3
Issue 4
Pg. 401-8
( 1988)
ISSN: 0890-6467 [Print] Switzerland |
PMID | 3067190
(Publication Type: Journal Article)
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Chemical References |
- Growth Substances
- Transforming Growth Factors
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Topics |
- Animals
- Bronchi
(cytology)
- Bronchial Neoplasms
(genetics, pathology)
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Cell Transformation, Neoplastic
(genetics, pathology)
- Epithelial Cells
- Gene Expression Regulation
- Genes, ras
- Growth Substances
(blood)
- Humans
- Mice
- Neoplasms, Experimental
(genetics, pathology)
- Transforming Growth Factors
(pharmacology)
- Tumor Cells, Cultured
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