Human bronchial epithelial cells neoplastically transformed by v-Ki-ras: altered response to inducers of terminal squamous differentiation.

Many human bronchial adenocarcinomas have been shown to contain an activated Ki-ras oncogene (Rodenhuis et al., N. Engl. J. Med. 317 929-935, 1987). To test the hypothesis that activated Ki-ras may be causally related to human bronchial carcinogenesis, v-Ki-ras oncogene was transferred into an established human bronchial epithelial cell line, BEAS-2B, by infection with Kirsten murine sarcoma virus (Ki-MSV) or by transfection with a plasmid containing the transforming region of Ki-MSV. These cells formed poorly differentiated adenocarcinomas in athymic nude mice. Cell lines established from these tumors expressed v-Ki-ras p21 protein and were highly tumorigenic. Whereas serum or transforming growth factor beta 1 induced the BEAS-2B cells at clonal density to undergo growth arrest and squamous differentiation, BEAS-2B cells containing activated ras genes were unaffected by transforming growth factor beta 1 and were mitogenically stimulated by serum.
AuthorsR R Reddel, Y Ke, M E Kaighn, L Malan-Shibley, J F Lechner, J S Rhim, C C Harris
JournalOncogene research (Oncogene Res) Vol. 3 Issue 4 Pg. 401-8 ( 1988) ISSN: 0890-6467 [Print] SWITZERLAND
PMID3067190 (Publication Type: Journal Article)
Chemical References
  • Growth Substances
  • Transforming Growth Factors
  • Animals
  • Bronchi (cytology)
  • Bronchial Neoplasms (genetics, pathology)
  • Cell Differentiation (drug effects)
  • Cell Division (drug effects)
  • Cell Transformation, Neoplastic (genetics, pathology)
  • Epithelial Cells
  • Gene Expression Regulation
  • Genes, ras
  • Growth Substances (blood)
  • Humans
  • Mice
  • Neoplasms, Experimental (genetics, pathology)
  • Transforming Growth Factors (pharmacology)
  • Tumor Cells, Cultured

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