Connexin43 mutations linked to skin disease have augmented hemichannel activity.
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| Abstract |
Mutations in the gene (GJA1) encoding connexin43 (Cx43) are responsible for several rare genetic disorders, including non-syndromic skin-limited diseases. Here we used two different functional expression systems to characterize three Cx43 mutations linked to palmoplantar keratoderma and congenital alopecia-1, erythrokeratodermia variabilis et progressiva, or inflammatory linear verrucous epidermal nevus. In HeLa cells and Xenopus oocytes, we show that Cx43-G8V, Cx43-A44V and Cx43-E227D all formed functional gap junction channels with the same efficiency as wild-type Cx43, with normal voltage gating and a unitary conductance of ~110 pS. In HeLa cells, all three mutations also localized to regions of cell-cell contact and displayed a punctate staining pattern. In addition, we show that Cx43-G8V, Cx43-A44V and Cx43-E227D significantly increase membrane current flow through formation of active hemichannels, a novel activity that was not displayed by wild-type Cx43. The increased membrane current was inhibited by either 2 mM calcium, or 5 µM gadolinium, mediated by hemichannels with a unitary conductance of ~250 pS, and was not due to elevated mutant protein expression. The three Cx43 mutations all showed the same gain of function activity, suggesting that augmented hemichannel activity could play a role in skin-limited diseases caused by human Cx43 mutations.
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| Authors | Miduturu Srinivas, Thomas F Jannace, Anthony G Cocozzelli, Leping Li, Nefeli Slavi, Caterina Sellitto, Thomas W White |
| Journal | Scientific reports (Sci Rep) Vol. 9 Issue 1 Pg. 19 (01 10 2019) ISSN: 2045-2322 [Electronic] England |
| PMID | 30631135 (Publication Type: Journal Article, Research Support, N.I.H., Extramural) |
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