Ascitic Bacterial Composition Is Associated With Clinical Outcomes in Cirrhotic Patients With Culture-Negative and Non-neutrocytic Ascites.

Abstract

Ascites bacterial burden is associated with poor clinical outcomes in patients with end-stage liver disease. However, the impact of ascitic microbial composition on clinical course was still not clear. In this study, the ascitic microbiota composition of 100 cirrhotic patients with culture-negative and non-neutrocytic ascites were researched with 16S rRNA pyrosequencing and enterotype-like cluster analysis. Results: By characterizing the ascitic microbial composition, two distinct microbial clusters were observed, Cluster 1 (86 patients) and Cluster 2 (14 patients). Cluster 1 showed lower microbial richness than Cluster 2. At the phylum level, Cluster 1 had greater abundance of Bacteroidetes and Firmicutes, but less abundance of Proteobacteria and Actinobacteria than Cluster 2. At the family level, family Bacteroidales S24-7 group, Prevotellaceae, Lachnospiraceae, Lactobacillaceae, Rikenellaceae, and Vibrionaceae were found over-represented in Cluster 1. And family Acetobacteraceae, Erysipelotrichaceae, Rickettsiaceae, and Streptococcaceae were found enriched in Cluster 2. The levels of plasma cytokine IL-17A, IL-7, and PDGF-BB were found significantly higher in Cluster 1 than in Cluster 2. There were four OTUs closely correlated with plasma cytokines, which were OTU 140 and OTU 271 (both from Bacteroidales S24-7 group), OTU 68 (Veillonellaceae), and OTU 53 (Helicobacteraceae). Patients from Cluster 1 showed significant higher short-term mortality than patients from Cluster 2. Conclusion: Our study demonstrated that the microbial composition of culture-negative and non-neutrocytic ascites in cirrhotic patients is associated with short-term clinical outcomes. The results here offer a rational for the identification of patients with high risk, and provide references for selective use of prophylactic methods.

• Authors: Yanfei Chen, Jing Guo, Ding Shi, Daiqiong Fang, Chunlei Chen, Lanjuan Li
• Journal: Frontiers in cellular and infection microbiology (Front Cell Infect Microbiol) Vol. 8 Pg. 420 ( 2018) ISSN: 2235-2988 [Electronic] Switzerland
• PMID: 30555804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Interleukin-17
• Interleukin-7
• RNA, Ribosomal, 16S
• Becaplermin

Topics

• Adult
• Aged
• Ascites (blood, microbiology)
• Ascitic Fluid (immunology, microbiology)
• Bacteria (classification, genetics, isolation & purification)
• Bacterial Translocation
• Becaplermin (blood)
• China
• Cluster Analysis
• Cytokines (blood)
• Female
• Humans
• Interleukin-17 (blood)
• Interleukin-7 (blood)
• Liver Cirrhosis (microbiology)
• Male
• Microbiota
• Middle Aged
• Peritonitis (microbiology)
• RNA, Ribosomal, 16S (genetics)