MiR-217 promotes cell proliferation and osteogenic differentiation of BMSCs by targeting DKK1 in steroid-associated osteonecrosis.

Abstract

MicroRNAs (miRNAs) have recently been recognized to play an important role in bone-associated diseases. This study aims to explore the expression profile and biological function of miR-217, which is known to be related to tumor cell proliferation and migration, to the proliferation and osteogenic differentiation of MSCs from the patients with steroid-associated osteonecrosis (ONFH). Bone marrow was obtained from the proximal femur of 10 patients with ONFH and 10 patients with femoral neck fractures. Bone marrow-derived mesenchymal stem cells (MSCs) were isolated and cultured. The expression profile, biological function of miR-217 and the interaction between miR-217 and DKK1 were assayed using cell viability measurement, western blot, Real-time PCR, luciferase reporter assay, Alizarin Red S (ARS) staining. We noted that the expression level of miR-217 was significantly decreased in the ONFH samples compared to the control samples (P < 0.0001). By targeting DKK1, miR-217 promoted nuclear translocation of β-catenin, increased expression of RUNX2, COL1A1 and obviously promoted the proliferation and differentiation of MSCs. Restoring the expression of DKK1 in the MSCs partially reversed the role of miR-217. These findings suggest that miR-217 promotes cell proliferation and osteogenic differentiation by inhibiting DKK1 during the development of steroid-associated osteonecrosis.

• Authors: Zhipeng Dai, Yi Jin, Jia Zheng, Ke Liu, Jiajun Zhao, Shanfeng Zhang, Fei Wu, Zhibo Sun
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 109 Pg. 1112-1119 (Jan 2019) ISSN: 1950-6007 [Electronic] France
• PMID: 30551361 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Chemical References

• Collagen Type I
• Collagen Type I, alpha 1 Chain
• Core Binding Factor Alpha 1 Subunit
• DKK1 protein, human
• Intercellular Signaling Peptides and Proteins
• MIRN217 microRNA, human
• MicroRNAs
• Steroids
• beta Catenin

Topics

• Aged
• Bone Marrow (physiology)
• Cell Differentiation (genetics)
• Cell Line
• Cell Proliferation (genetics)
• Cell Survival (genetics)
• Collagen Type I (genetics)
• Collagen Type I, alpha 1 Chain
• Core Binding Factor Alpha 1 Subunit (genetics)
• Female
• Humans
• Intercellular Signaling Peptides and Proteins (genetics)
• Male
• Mesenchymal Stem Cells (physiology)
• MicroRNAs (genetics)
• Middle Aged
• Osteogenesis (genetics)
• Steroids (metabolism)
• beta Catenin (genetics)