Abstract |
Paclitaxel- betulinic acid hybrid nanosuspensions (PTX-BA-NP) with increased anti- breast cancer activity were developed. The resultant nanosuspensions (NP) had a mean particle size of 282.54 ± 5.4 nm, a polydispersity index of approximately 0.242 ± 0.02, a zeta potential of -19.7 ± 0.19 mV and a redispersibility index of 103.3 ± 0.01%. The cumulative dissolution percentage of PTX coarse powder and PTX-BA-NP dried powder at 60 min were 15.4% and 90.8%, respectively. MCF-7 cell-based testing showed that treatment with PTX-BA-NP led to more PTX-BA-NP accumulation in the cytoplasm of breast cancer cells, less cell cycle arrest in the G2-M phase, more cell cycle arrest in the G0-G1 phase, more apoptosis-induced cell death and stronger inhibition of cell migration than paclitaxel nanosuspensions (PTX-NP). Biodistribution studies showed that tumor accumulation levels at 12 h in the PTX-BA-NP group were approximately 2.67- and 2.33-fold higher than the levels in the Taxol® and PTX-NP groups, respectively. In vivo antitumor efficacy demonstrated that PTX-BA-NP exerted the strongest tumor inhibition among the four groups, with a tumor inhibition rate of 47.79 ± 2.28%, followed by PTX-NP (35.05 ± 5.55%), Taxol® (22.67 ± 6.01%) and betulinic acid nanosuspension (BANP) (14.38 ± 6.02%). These findings indicate that PTX-BA-NP holds great promise for breast cancer therapy.
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Authors | Ruoning Wang, Mengmeng Yang, Guoyuan Li, Xin Wang, Zhenhai Zhang, Hongzhi Qiao, Jun Chen, Zhipeng Chen, Xiaobin Cui, Junsong Li |
Journal | Colloids and surfaces. B, Biointerfaces
(Colloids Surf B Biointerfaces)
Vol. 174
Pg. 270-279
(Feb 01 2019)
ISSN: 1873-4367 [Electronic] Netherlands |
PMID | 30469048
(Publication Type: Journal Article)
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Copyright | Copyright © 2018 Elsevier B.V. All rights reserved. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Pentacyclic Triterpenes
- Triterpenes
- Paclitaxel
- Betulinic Acid
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Topics |
- Animals
- Antineoplastic Agents, Phytogenic
(chemistry, pharmacology)
- Apoptosis
(drug effects)
- Breast Neoplasms
(drug therapy, pathology)
- Cell Cycle
(drug effects)
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Female
- Humans
- MCF-7 Cells
- Mammary Neoplasms, Experimental
(drug therapy, pathology)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Nanoparticles
(chemistry)
- Paclitaxel
(chemistry, pharmacology)
- Pentacyclic Triterpenes
- Structure-Activity Relationship
- Tissue Distribution
- Triterpenes
(chemistry, pharmacology)
- Betulinic Acid
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