Biomolecular analysis of a defective nontransforming Epstein-Barr virus (EBV) from a patient with chronic active EBV infection.

Abstract

A virus recovered from the saliva of a child with chronic active Epstein-Barr virus (EBV) infection for 8 years was shown to induce EBV early antigen (EBV-EA) in Raji cells and to be expressed into EBV-EA in fresh EBV-negative peripheral blood leukocytes. However, it did not replicate its DNA. Oropharyngeal epithelial cells scraped from recurrent mouth lesions were similarly positive for EBV-EA. DNA extracted from these cells and digested with BamHI contained a 6-kilobase-pair fragment homologous to BamHI fragment V and B1 EBV DNA probes. Furthermore, Southern blots of the BamHI and EcoRI digests of the DNA extracted from the cell lines of the patient (transformed with EBV strain B95-8) and of her mother (spontaneous) revealed, in addition to the expected BamHI G, H, H2, and B1 fragments used as probes, additional shorter ones of a presumably endogenous defective virus.

• Authors: C Alfieri, J H Joncas
• Journal: Journal of virology (J Virol) Vol. 61 Issue 10 Pg. 3306-9 (Oct 1987) ISSN: 0022-538X [Print] United States
• PMID: 3041050 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• DNA, Viral
• Epstein-Barr virus early antigen
• DNA Restriction Enzymes
• Deoxyribonuclease BamHI
• Deoxyribonuclease EcoRI

Topics

• Antigens, Viral (biosynthesis)
• Cell Line
• Cell Transformation, Viral
• Child
• Chronic Disease
• DNA Replication
• DNA Restriction Enzymes
• DNA, Viral (analysis)
• Defective Viruses (genetics)
• Deoxyribonuclease BamHI
• Deoxyribonuclease EcoRI
• Female
• Genes, Viral
• Herpesviridae Infections (microbiology)
• Herpesvirus 4, Human (genetics, immunology)
• Humans
• Nucleic Acid Hybridization
• Virus Replication