Vitamins E and K induce aryl hydrocarbon hydroxylase activity in human cell cultures.

Abstract

Two fat soluble vitamins, Vitamins E and K, when added into culture medium, were found to increase aryl hydrocarbon hydroxylase activity in human cultured cells. The extent of induction in a hepatoma-derived cell line (Hep G2) by these vitamins is of similar magnitude to those cells receiving benz[a]anthracene; whereas in a mammary tumor-derived cell line (MCF-7), benz[a]anthracene is the best inducer for the hydroxylase activity. The increase of the hydroxylase activity is associated with increased levels of a specific mRNA coding for polynuclear aromatic hydrocarbons-induced form of cytochrome P-450 with Vitamins E and K treatment. The size of the induced mRNA is 3.3 kilobase which is the same as that of benz[a]anthracene treatment.

• Authors: Y T Chen, J H Ding
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 143 Issue 3 Pg. 863-71 (Mar 30 1987) ISSN: 0006-291X [Print] United States
• PMID: 3032186 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Vitamin K
• Vitamin E
• Aryl Hydrocarbon Hydroxylases

Topics

• Aryl Hydrocarbon Hydroxylases (biosynthesis)
• Breast Neoplasms (enzymology)
• Carcinoma, Hepatocellular (enzymology)
• Cell Line
• Enzyme Induction (drug effects)
• Humans
• Kinetics
• Liver Neoplasms
• Vitamin E (pharmacology)
• Vitamin K (pharmacology)