Glucose regulates preproinsulin messenger RNA levels in a clonal cell line of simian virus 40-transformed B cells.

Abstract

In HIT-T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co-migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2-20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose-dependent manner. Glucose-stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.

• Authors: P Hammonds, P N Schofield, S J Ashcroft
• Journal: FEBS letters (FEBS Lett) Vol. 213 Issue 1 Pg. 149-54 (Mar 09 1987) ISSN: 0014-5793 [Print] England
• PMID: 3030804 (Publication Type: Journal Article)

Chemical References

• Insulin
• Protein Precursors
• RNA, Messenger
• Dactinomycin
• Colforsin
• preproinsulin
• Proinsulin
• Theophylline
• Protein Kinases
• Glucose
• Bombesin

Topics

• Animals
• B-Lymphocytes (metabolism)
• Bombesin (pharmacology)
• Cell Line
• Cell Transformation, Viral
• Clone Cells
• Colforsin (pharmacology)
• Cricetinae
• Dactinomycin (pharmacology)
• Dose-Response Relationship, Drug
• Glucose (pharmacology)
• Insulin
• Mesocricetus
• Proinsulin (genetics)
• Protein Kinases (metabolism)
• Protein Precursors (genetics)
• RNA, Messenger (metabolism)
• Simian virus 40
• Theophylline (pharmacology)