Enzyme markers of collagen synthesis in carbon tetrachloride-induced fibrosis and during colchicine modification of CCl4-induced liver injury.

Abstract

Serum galactosylhydroxylysyl glucosyltransferase (S-Glu-Gal-Hyl-Tase), liver galactosylhydroxylysyl glucosyltransferase (L-Glu-Gal-Hyl-Tase), liver hydroxylysyl galactosyltransferase (L-Gal-Hyl-Tase), and liver prolyl hydroxylase (L-PH) activities were measured in rats during the development of CCl4-induced cirrhosis (0.2 ml of 33% CCl4 in light mineral oil two times weekly for 10 weeks followed by 6 weeks of no treatment). Serum and liver markers of collagen synthesis increased in a time-dependent manner reaching maximum activity at 6 weeks (S-Glu-Gal-Hyl-Tase, two times; L-PH, two times). These enzyme levels returned to normal during the 4-week recovery period. In a separate 4-week experiment, colchicine (10 micrograms/rat/day) was administered with CCl4. Colchicine prevented the increase in S-Glu-Gal-Hyl-Tase, L-Glu-Gal-Hyl-Tase, and L-Gal-Hyl-Tase induced by CCl4 and resulted in a smaller increase in L-PH. These results demonstrate that S-Glu-Gal-Hyl-Tase elevation occurs following CCl4 because of increased liver collagen synthetic activity and the hepatocellular injury produced by CCl4.

• Authors: D M Bolarin, K Barker, G C Fuller
• Journal: Experimental and molecular pathology (Exp Mol Pathol) Vol. 46 Issue 2 Pg. 145-52 (Apr 1987) ISSN: 0014-4800 [Print] Netherlands
• PMID: 3030797 (Publication Type: Journal Article)

Chemical References

• Collagen
• Procollagen-Proline Dioxygenase
• Galactosyltransferases
• Glucosyltransferases
• UDPgalactose-collagen galactosyltransferase
• UDP glucose-collagen glucosyltransferase
• Colchicine

Topics

• Animals
• Carbon Tetrachloride Poisoning (metabolism)
• Colchicine (pharmacology)
• Collagen (biosynthesis)
• Galactosyltransferases (metabolism)
• Glucosyltransferases (blood, metabolism)
• Liver (enzymology)
• Male
• Procollagen-Proline Dioxygenase (metabolism)
• Rats
• Time Factors