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Direct extraction and assay of bone tissue collagenase and its relation to parathyroid-hormone-induced bone resorption.

Abstract
A method has been developed for the quantitative extraction of collagenase from as little as one 19-day-fetal-mouse calvarium. About 20-40 munits of collagenase are extracted per mg of tissue, all in a latent form that, after proper activation, shows the typical properties of mammalian collagenase. Culturing the calvaria for 2 days with parathyroid hormone (PTH) increases their procollagenase content up to 3-fold and induces bone resorption. Both PTH effects are prevented by cycloheximide, but not by indomethacin. Calcitonin inhibits resorption without affecting the PTH-induced procollagenase synthesis. The role of this synthesis is discussed in relation to the mechanisms of bone resorption.
AuthorsY Eeckhout, J M Delaissé, G Vaes
JournalThe Biochemical journal (Biochem J) Vol. 239 Issue 3 Pg. 793-6 (Nov 01 1986) ISSN: 0264-6021 [Print] England
PMID3030273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Calcitonin
  • Cycloheximide
  • Microbial Collagenase
  • Indomethacin
Topics
  • Animals
  • Bone Resorption (drug effects)
  • Bone and Bones (drug effects, enzymology)
  • Calcitonin (pharmacology)
  • Cycloheximide (pharmacology)
  • Indomethacin (pharmacology)
  • Mice
  • Microbial Collagenase (antagonists & inhibitors, isolation & purification, metabolism)
  • Organ Culture Techniques

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