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Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ).

Abstract
The glutamine permease operon encoding the high-affinity transport system of glutamine in Escherichia coli could be cloned in one of the mini F plasmids, but not in pBR322 or pACYC184, by selection for restoration of the Gln+ phenotype, the ability to utilize glutamine as a sole carbon source. We determined the nucleotide sequence of the glutamine permease operon, which contains the structural gene of the periplasmic glutamine-binding protein (glnH), and indispensable component of the permease activity. The N-terminal amino acid sequence and the overall amino acid composition of the purified glutamine-binding protein were in good agreement with those predicted from the nucleotide sequence, if the N-terminal 22 amino acid residues were discounted. The latter comprised two Lys residues (nos. 2 and 6) followed by 16 hydrophobic amino acid residues and was assumed to be a signal peptide for transport into the periplasmic space. There were two additional reading frames (glnP and glnQ) downstream of glnH sharing a common promoter. It was concluded that the glnP and glnQ proteins as well as the glnH protein are essential for glutamine permease activity.
AuthorsT Nohno, T Saito, J S Hong
JournalMolecular & general genetics : MGG (Mol Gen Genet) Vol. 205 Issue 2 Pg. 260-9 (Nov 1986) ISSN: 0026-8925 [Print] Germany
PMID3027504 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Amino Acid Transport Systems, Basic
  • Carrier Proteins
  • Escherichia coli Proteins
  • Membrane Proteins
  • Membrane Transport Proteins
  • glnP protein, E coli
  • glutamine transport proteins
  • glutamine permease
  • DNA Restriction Enzymes
Topics
  • Amino Acid Transport Systems, Basic
  • Base Sequence
  • Carrier Proteins (genetics)
  • Cloning, Molecular
  • DNA Restriction Enzymes
  • Escherichia coli (genetics)
  • Escherichia coli Proteins
  • Genes
  • Genes, Bacterial
  • Genotype
  • Membrane Proteins
  • Membrane Transport Proteins (genetics)
  • Operon
  • Plasmids

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