[Effect of avitaminosis B6 in mice on the function of the T-lymphocytes in vitro].

Abstract

The influence of different degrees of avitaminosis B6 in mice on the cytolytic activity of T-lymphocytes measured by the amount of Na2Cr51O4 released from the lysed target cells was studied on a model of primary immune response in a mixed lymphocyte culture in vitro. Keeping of the animals for 3 weeks on pyridoxine-free diet failed to influence the capacity of lymphocytes to proliferate in vitro and their cytolytic activity. In animals on pyridoxine-free diet for 45 days the amount of pyridoxal 5(1)-phosphate in the spleen decreased by 55% in comparison with control. Lymphocytes obtained from these animals and cultivated in vitro had a markedly decreased capacity to 3H-thymidine incorporation into DNA in response to alloantigen. The cytolytic activity of these lymphocytes also diminished. The capacity of various pyridoxine forms to restore T-lymphocyte functions disturbed by avitaminosis B6 was studied.

• Authors: A V Sergeev, S N Bykovskaia, L M Luchanskaia, A A Ivanova, M O Raushenbakh
• Journal: Biulleten' eksperimental'noi biologii i meditsiny (Biull Eksp Biol Med) Vol. 84 Issue 8 Pg. 185-8 (Aug 1977) ISSN: 0365-9615 [Print] Russia (Federation)
• Vernacular Title: Vliianie avitaminoza B6 u mysheĭ na funktsiiu T-limfotsitov in vitro.
• PMID: 302724 (Publication Type: Comparative Study, English Abstract, Journal Article)

Topics

• Animals
• Cell Division
• Cytotoxicity, Immunologic
• Female
• In Vitro Techniques
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• T-Lymphocytes (immunology)
• Time Factors
• Vitamin B 6 Deficiency (immunology)