UPLC-MS/MS determination of flavokawain B, a novel anti-tumor chemotherapeutic agent in rat plasma and its application to a pharmacokinetic study in rats.

Abstract

A sensitive, selective and rapid ultra-performance liquid chromatography/tandem mass spectrometry method was developed and validated for the quantification of flavokawain B in rat plasma using myrislignan as an internal standard. Sample preparation was accomplished through a protein precipitation extraction process. Chromatographic resolution of flavokawain B and the IS was achieved on an Agilent XDB-C18 column (2.1 × 100 mm, 1.8 μm) using a gradient mobile phase comprising 0.1% formic acid in water and acetonitrile delivered at a flow rate of 0.5 mL/min. Flavokawain B and the IS eluted at 3.27 and 1.96 min, respectively. The total chromatographic run time was 6.0 min. A linear response function was constructed in the concentration range 0.524-1048 ng/mL. Method validation was performed as per the US Food and Drug Administration guidelines and the results met the acceptance criteria. Intra- and inter-day accuracy and precision were in the ranges of -14.3-13.2 and 3.4-11.8%, respectively. Flavokawain B was demonstrated to be stable under various stability conditions. This method has been applied to a pharmacokinetic study in rats.

• Authors: Xueqin Yang, Tao Zan, Huiyu Yan, Baohua Liu
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 33 Issue 2 Pg. e4391 (Feb 2019) ISSN: 1099-0801 [Electronic] England
• PMID: 30238480 (Publication Type: Journal Article)
• Copyright: © 2018 John Wiley & Sons, Ltd.

Chemical References

• Antineoplastic Agents
• Flavonoids
• flavokawain B

Topics

• Animals
• Antineoplastic Agents (blood, chemistry, pharmacokinetics)
• Chromatography, High Pressure Liquid (methods)
• Drug Stability
• Flavonoids (blood, chemistry, pharmacokinetics)
• Linear Models
• Male
• Rats
• Rats, Sprague-Dawley
• Reproducibility of Results
• Sensitivity and Specificity
• Tandem Mass Spectrometry (methods)