Abstract | BACKGROUND: METHODS:
Abdominal aortic aneurysms were induced in WT C57BL/6 male mice (n = 9-18/group), using an established topical elastase abdominal aortic aneurysm model. Osmotic pumps were implanted in mice 5 days before operation to create the model, administering either low dose (0.125 µg/day tamsulosin), high dose (0.250µg/day tamsulosin), or vehicle treatments with and without topical application of elastase. Blood pressures were measured preoperatively and on postoperative days 0, 3, 7, and 14. On postoperative day 14, aortic diameter was measured before harvest. Sample aortas were prepared for histology and cytokine analysis. RESULTS: Measurements of systolic blood pressure did not differ between groups. Mice treated with the low dose of tamsulosin and with the high dose of tamsulosin showed decreased aortic diameter compared with vehicle-treated control (93% ± 24 versus 94% ± 30 versus 132% ± 24, respectively; P = .0003, P = .0003). Cytokine analysis demonstrated downregulation of pro-inflammatory cytokines in both treatment groups compared with the control (P < .05). Histology exhibited preservation of elastin in both low- and high-dose tamsulosin-treated groups (P = .0041 and P = .0018, respectively). CONCLUSION:
|
Authors | William G Montgomery, Michael D Spinosa, J Michael Cullen, Morgan D Salmon, Gang Su, Taryn Hassinger, Ashish K Sharma, Guanyi Lu, Anna Fashandi, Gorav Ailawadi, Gilbert R Upchurch Jr |
Journal | Surgery
(Surgery)
Vol. 164
Issue 5
Pg. 1087-1092
(11 2018)
ISSN: 1532-7361 [Electronic] United States |
PMID | 30174141
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Copyright | Copyright © 2018 Elsevier Inc. All rights reserved. |
Chemical References |
- Adrenergic alpha-1 Receptor Antagonists
- Cytokines
- Elastin
- Pancreatic Elastase
- Tamsulosin
|
Topics |
- Adrenergic alpha-1 Receptor Antagonists
(pharmacology, therapeutic use)
- Animals
- Aorta, Abdominal
(drug effects, pathology)
- Aortic Aneurysm, Abdominal
(chemically induced, pathology, prevention & control)
- Blood Pressure
(drug effects)
- Cytokines
(metabolism)
- Disease Models, Animal
- Down-Regulation
(drug effects)
- Drug Evaluation, Preclinical
- Elastin
(metabolism)
- Humans
- Male
- Mice
- Mice, Inbred C57BL
- Pancreatic Elastase
(toxicity)
- Tamsulosin
(pharmacology, therapeutic use)
- Treatment Outcome
|