Amentoflavone Inhibits Hepatocellular Carcinoma Progression Through Blockage of ERK/NF-ĸB Activation.

Abstract

AIM:

The aim of the present study was to confirm therapeutic efficacy and find probable mechanism of action of amentoflavone in hepatocellular carcinoma (HCC) in vivo.

MATERIALS AND METHODS:

Luciferase reporter vector pGL4.50_transfected SK-Hep1 (SK-Hep1/luc2) tumor-bearing mice were treated with vehicle or amentoflavone (100 mg/kg/day by gavage) for 14 days. Tumor growth, amentoflavone toxicity, and extracellular signal-regulated kinase (ERK)/nuclear factor-kappaB (NF-ĸB) signaling in tumor progression were evaluated with digital caliper, bioluminescence imaging, computed tomography, body weight, pathological examination of liver, and immunohistochemistry staining.

RESULTS:

Amentoflavone significantly inhibited tumor growth, ERK/NF-ĸB activation, and expression of tumor progression-associated proteins as compared to vehicle-treated group. In addition, body weight and liver morphology of mice were not influenced by amentoflavone treatment.

CONCLUSION:

These results suggest that amentoflavone inhibits HCC progression through suppression of ERK/NF-ĸB signaling.

• Authors: Kun-Ching Lee, Wei-Ting Chen, Yu-Chang Liu, Song-Shei Lin, Fei-Ting Hsu
• Journal: In vivo (Athens, Greece) (In Vivo) 2018 Sep-Oct Vol. 32 Issue 5 Pg. 1097-1103 ISSN: 1791-7549 [Electronic] Greece
• PMID: 30150431 (Publication Type: Journal Article)
• Copyright: Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Antineoplastic Agents
• Biflavonoids
• NF-kappa B
• amentoflavone
• Extracellular Signal-Regulated MAP Kinases

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Biflavonoids (pharmacology)
• Carcinoma, Hepatocellular (drug therapy, metabolism, pathology)
• Cell Line, Tumor
• Disease Models, Animal
• Disease Progression
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Genes, Reporter
• Humans
• Immunohistochemistry
• Liver Neoplasms (drug therapy, metabolism, pathology)
• Mice
• Molecular Imaging
• NF-kappa B (metabolism)
• Signal Transduction (drug effects)
• Tumor Burden (drug effects)
• Xenograft Model Antitumor Assays