Human prion protein cDNA: molecular cloning, chromosomal mapping, and biological implications.

Abstract

A human complementary DNA whose protein product is considered to be the major component of scrapie-associated fibrils in Creutzfeldt-Jakob disease, kuru, and Gerstmann-Straussler syndrome has been identified and characterized. The extensive homology of this gene sequence to the hamster PrP 27- to 30-kilodalton prion protein complementary DNA clone, and its existence as a single copy in the human genome, leads to the conclusion that this is the human prion gene. This human prion gene has been mapped to human chromosome 20, negating a direct link between the prion protein and Down's syndrome or the amyloid of Alzheimer's disease.

• Authors: Y C Liao, R V Lebo, G A Clawson, E A Smuckler
• Journal: Science (New York, N.Y.) (Science) Vol. 233 Issue 4761 Pg. 364-7 (Jul 18 1986) ISSN: 0036-8075 [Print] United States
• PMID: 3014653 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Prions
• Viral Proteins
• DNA
• DNA Restriction Enzymes

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Chromosome Mapping
• Chromosomes, Human, 19-20
• Chromosomes, Human, 21-22 and Y
• Cloning, Molecular
• Creutzfeldt-Jakob Syndrome (genetics, microbiology)
• Cricetinae
• DNA (analysis)
• DNA Restriction Enzymes (metabolism)
• Humans
• Prions (genetics)
• Viral Proteins (analysis)