Abstract |
Persistent infections (Pi) were established in two host-cell systems [Madin-Darby bovine kidney (MDBK) and Madin-Darby canine kidney (MDCK)] with Sendai virus and three strains of NDV, to test the influence of different viruses and host-cell systems. Virus was recovered from the persistently infected cells. An RNA- ts mutant was recovered from a Pi of MDBK cells, but no Pi could be established in MDCK cells with the three strains of NDV. Additionally, the Pi was established exclusively by a virulent strain, NDV-Milano. On the other hand, Sendai virus could establish Pi in MDBK and MDCK cell-systems. Several ts mutants were recovered from "late" passages of Pi, and from an accidental infection, a ts mutant with an altered P polypeptide. Ten other ts mutants were tested, however, the specific ts lesion could not be identified. From three Pi in MDCK cells, host range mutants (ts-f1, ts-f2, and ts-f3) were recovered. One of the mutants (ts-f1) has an altered M (matrix) protein. The host range mutants undergo a productive infection in MDBK and MDCK cells, which are nonpermissive for wild type Sendai virus. The possible significance of the results are discussed.
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Authors | P Lawton, Z Karimi, L Mancinelli, J T Seto |
Journal | Archives of virology
(Arch Virol)
Vol. 89
Issue 1-4
Pg. 225-33
( 1986)
ISSN: 0304-8608 [Print] Austria |
PMID | 3013129
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Cattle
- Cell Line
- Chick Embryo
- Culture Techniques
(methods)
- Electrophoresis, Polyacrylamide Gel
- Fibroblasts
- Kidney
- Newcastle disease virus
(growth & development)
- Parainfluenza Virus 1, Human
(growth & development)
- Species Specificity
- Viral Proteins
(analysis)
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