Theabrownin suppresses in vitro osteoclastogenesis and prevents bone loss in ovariectomized rats.

Abstract

Drinking tea exhibits beneficial effects on bone health and may protect against osteoporosis, particularly in postmenopausal women. Theabrownin (TB) is the main component responsible for the biological activities of Pu-erh tea, but whether it possesses anti-osteoporotic potential remains unknown. Here we investigated the in vitro and in vivo anti-osteoporotic effects of TB in the RAW 264.7 cell line and ovariectomized (OVX) rats, respectively. Our in vitro studies showed that TB significantly suppressed RANKL-induced osteoclastogenesis and the expression of related marker proteins, including NFATc1, TRAP, c-Fos, and cathepsin K. In vivo studies showed that TB treatment effectively ameliorated blood biochemical parameters, organ weights and organ coefficients in OVX rats. In addition, TB treatment significantly improved femoral bone mineral density (BMD) and biomechanical properties. What's more, TB treatment strikingly ameliorated bone microarchitecture in OVX rats because of increased cortical bone thickness and trabecular bone area in the femur. Our study therefore demonstrated that TB can inhibit RANKL-induced osteoclastogenesis in vitro and prevent bone loss in ovariectomized rats. Consequently, TB has a promising potential in postmenopausal osteoporosis treatment.

• Authors: Titi Liu, Zemin Xiang, Fei Chen, Dan Yin, Yewei Huang, Jing Xu, Lihong Hu, Huanhuan Xu, Xuanjun Wang, Jun Sheng
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 106 Pg. 1339-1347 (Oct 2018) ISSN: 1950-6007 [Electronic] France
• PMID: 30119205 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier Masson SAS. All rights reserved.

Chemical References

• Biomarkers
• Bone Density Conservation Agents
• RANK Ligand
• Tnfsf11 protein, mouse
• theabrownin
• Catechin

Topics

• Animals
• Biomarkers (blood)
• Bone Density Conservation Agents (pharmacology)
• Bone Remodeling (drug effects)
• Catechin (analogs & derivatives, pharmacology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Female
• Femur (drug effects, metabolism, pathology)
• Humans
• Mice
• Osteoclasts (drug effects, metabolism, pathology)
• Osteogenesis (drug effects)
• Osteoporosis, Postmenopausal (metabolism, pathology, prevention & control)
• Ovariectomy
• RANK Ligand (pharmacology)
• RAW 264.7 Cells
• Rats, Wistar