Stimulatory guanine nucleotide binding protein activity in the erythrocyte membrane of patients with pseudohypoparathyroidism type I and related disorders.

Abstract

The activity of stimulatory guanine nucleotide regulatory protein (Ns) in the erythrocyte membrane was assayed by the reconstitution method using plasma membrane of cyc S49 mouse lymphoma cells in 18 patients with type I pseudohypoparathyroidism (PHP-I), 2 with pseudopseudohypoparathyroidism (PPHP) and 30 normal subjects, in parallel with other clinical parameters. The Ns activity as expressed by per cent of pooled standard (mean +/- SE) was 78.9 +/- 6.1 in PHP-I patients, which was significantly lower (P less than 0.01) than the value in normal subjects, 99.5 +/- 2.4. In PHP-I patients, the Ns activities (Y) were in significant correlation with three clinical parameters examined (X), i.e., with body height in standard deviation score from the mean of the normal population at the corresponding age, Y = 89.4 + 10.4X (r = 0.616, P less than 0.01); with urinary cAMP excretion in relation to creatinine [cAMP(nmol)/Cr(mg)], Y = 56.3 + 7.2X (r = 0.501, P less than 0.05); and with TSH levels in plasma (microU/ml), Y = 129 - 3X (r = 0.639, P less than 0.01). The Ns activities of PPHP were as low as 53.8 and 60.0. The decrease of Ns activity in the cell membrane may be implicated in the development of the clinical symptoms such as short stature, decrease in urinary excretion of cAMP and latent or manifest primary hypothyroidism in PHP-I and possibly in skeletal abnormality in PPHP.

• Authors: T Saito, Y Akita, H Fujita, Y Furukawa, Y Tsuchiya, T Yasuda, M Yamamoto, T Kitagawa, Y Nakagawa, A Takehiro
• Journal: Acta endocrinologica (Acta Endocrinol (Copenh)) Vol. 111 Issue 4 Pg. 507-15 (Apr 1986) ISSN: 0001-5598 [Print] Denmark
• PMID: 3010621 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Thyroid Hormones
• Adenosine Diphosphate
• Adenosine Triphosphate
• Cyclic AMP
• GTP-Binding Proteins

Topics

• Adenosine Diphosphate (analysis)
• Adenosine Triphosphate (analysis)
• Adolescent
• Adult
• Body Height
• Cyclic AMP (urine)
• Erythrocyte Membrane (metabolism)
• Female
• GTP-Binding Proteins (blood)
• Humans
• Hypoparathyroidism (blood)
• Hypothyroidism (blood)
• Male
• Methods
• Pseudohypoparathyroidism (blood)
• Pseudopseudohypoparathyroidism (blood)
• Thyroid Hormones (blood)