Mitomycin C-augmented production of I-A antigen-positive macrophages in tumor vaccine-primed mice.

Abstract

Intraperitoneal inoculation of L1210 murine leukemia cell vaccine increased I-Ad antigen-positive and -negative macrophages in the peritoneal cavity of histocompatible mice. Mitomycin C, a poor producer of I-Ad antigen-positive macrophages, selectively augmented the production of I-Ad antigen-positive macrophages in L1210 vaccine-primed mice, since it did not augment the production of non-macrophage cells. Since our previous study showed that the priming of mice with L1210 vaccine and mitomycin C induced augmented antitumor response, we tested the feasibility of the association of mitomycin C-augmented I-Ad antigen-positive macrophages with the augmented antitumor response. Prostaglandin E2 suppressed the antitumor response in L1210 vaccine- and mitomycin C-primed mice and, consistent with this, it inhibited the production of I-Ad antigen-positive macrophages, although it inhibited the production of non-macrophage cells as well. This hypothesis was further tested by the use of silica. When L1210 vaccine- and mitomycin C-primed mice were further given silica on the day of and one day after live L1210 inoculation, their antitumor response was strongly suppressed, while a kinetic analysis of the cellularity of peritoneal cells showed that administration of silica resulted in a decrease of I-Ad antigen-positive macrophages, but not I-Ad antigen-negative macrophages or non-macrophage cells in these mice. These results strongly suggest, though they do not prove, the association of mitomycin C-augmented production of I-Ad antigen-positive macrophages with the antitumor response in L1210 vaccine- and mitomycin C-primed mice.

• Authors: T Kataoka, T Yamamoto, K Honjo, T Murayama
• Journal: Japanese journal of cancer research : Gann (Jpn J Cancer Res) Vol. 77 Issue 3 Pg. 305-11 (Mar 1986) ISSN: 0910-5050 [Print] Japan
• PMID: 3009373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Histocompatibility Antigens Class II
• Mitomycins
• Prostaglandins E
• Vaccines
• Mitomycin
• Silicon Dioxide
• Dinoprostone

Topics

• Animals
• Antigen-Presenting Cells (drug effects, immunology)
• Dinoprostone
• Histocompatibility Antigens Class II (analysis)
• Leukemia L1210 (immunology)
• Macrophages (drug effects, immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred DBA
• Mitomycin
• Mitomycins (pharmacology)
• Prostaglandins E (pharmacology)
• Silicon Dioxide (pharmacology)
• Vaccines (immunology)