Vasoregulin, a glucocorticoid-inducible vascular permeability inhibitory protein.

Abstract

A vascular permeability inhibitory protein 'Vasoregulin' was induced by dexamethasone and other glucocorticoids in Namalva cells. Precipitates produced by trichloroacetic acid from cultured supernatant (crude released vasoregulin) suppressed serotonin-induced paw edema of mice (ID30 = 750 micrograms/mouse) when dosed 30 min. before serotonin. Carrageenan paw edema of rats was also suppressed (ID30 = 9 mg/rat, s.c. and i.p., not by oral dosing) when the compound was injected at the same time as carrageenan and the edema determined after 3 hrs. The possible effect of glucocorticoid itself being contained in the vasoregulin preparation was excluded. The presence of Cu, Zn-superoxide dismutase (SOD), spermidine and spermine also gave good yields of vasoregulin in culture; SOD protected the inactivation of vasoregulin from superoxide radical in vitro. Vasoregulin partially purified by Sephadex G-75 columns had no malonaldehyde-formation inhibitory capacity. Thus, vasoregulin is a glucocorticoid-inducible anti-inflammatory protein which differs from lipocortin.

• Authors: Y Oyanagui, S Suzuki
• Journal: Agents and actions (Agents Actions) Vol. 17 Issue 3-4 Pg. 270-7 (Jan 1986) ISSN: 0065-4299 [Print] Switzerland
• PMID: 3008526 (Publication Type: Journal Article)

Chemical References

• Glucocorticoids
• Neoplasm Proteins
• Prostaglandins
• Proteins
• vasoregulin
• Superoxides
• Serotonin
• Carrageenan
• Phospholipases A

Topics

• Animals
• Capillary Permeability (drug effects)
• Carrageenan
• Cells, Cultured
• Edema (physiopathology)
• Glucocorticoids (pharmacology)
• Humans
• Male
• Mice
• Mice, Inbred ICR
• Neoplasm Proteins
• Phospholipases A (antagonists & inhibitors)
• Prostaglandins (biosynthesis)
• Proteins (analysis, isolation & purification, pharmacology)
• Rats
• Serotonin
• Superoxides (metabolism)
• Time Factors