Abstract |
Hybridomas secreting monoclonal antibodies that reacted with the B6 radiation leukemia virus (RadLV)-induced leukemia B6RV2 were produced by fusion of BALB/c NS-1 myeloma cells with spleen cells from (BALB/c X B6)F1 mice immunized with B6RV2. By direct and absorption analyses with 28 B6 and BALB/c leukemias, the monoclonal antibodies NU7-4 and NU7-99 were shown to react only with B6RV2, indicating that they recognized an individually distinct antigen on B6RV2 that was identified previously with conventional (BALB/c X B6)F1 anti-B6RV2 serum. Another monoclonal antibody, NU1-132, showed relatively restricted reactivity with B6 RadLV leukemias. These three monoclonal antibodies all precipitated material of approximately 80,000 daltons, which is the same size as that precipitated by anti-xenotropic MuLV gp70 serum. Sequential immunoprecipitation analysis revealed that the molecules precipitated by NU7-4 were not removed by pretreatment of NU7-99 or NU1-132 and that the molecules precipitated by NU7-99 were not removed by NU7-4 or NU1-132. The molecules precipitated by NU1-132 were partially removed by pretreatment with NU7-4, but not with NU7-99. The molecules precipitated by these three monoclonal antibodies were removed by pretreatment with anti-xenotropic gp70. These results suggested heterogeneity of the xenotropic MuLV gp70-related molecules expressed on B6RV2 and a possible relation between serologically defined unique tumor antigens and gp70-related molecules.
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Authors | E Nakayama, A Uenaka, A B DeLeo, E Stockert, Y Obata, R Ueda, Y Inui |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 136
Issue 9
Pg. 3502-7
(May 01 1986)
ISSN: 0022-1767 [Print] United States |
PMID | 3007622
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Surface
- Antigens, Viral, Tumor
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Topics |
- Animals
- Antibodies, Monoclonal
(analysis)
- Antibody Specificity
- Antigen-Antibody Reactions
- Antigens, Surface
(immunology)
- Antigens, Viral, Tumor
(genetics, immunology, isolation & purification)
- Binding Sites, Antibody
- Binding, Competitive
- Electrophoresis, Polyacrylamide Gel
- Leukemia Virus, Murine
(genetics, immunology)
- Leukemia, Radiation-Induced
(immunology, microbiology)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Precipitin Tests
- Rabbits
- Rats
- Retroviridae
(immunology)
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