[Energy characteristics of the transport of monovalent cations in ascites tumor cells].

Abstract

The intensity of O2 adsorption by ascite tumour cells does not practically depend on the monovalent cation concentration gradient between the cells and the incubation medium, whereas the rate of glycolysis decreases simultaneously with the diminution of the concentration gradient. In synchronized cultures at the beginning of the mitotic cycle, the bulk of ATP resynthesized via glycolysis is utilized for the synthesis of biopolymers, whereas that at the end of the S-phase and in the G2-phase--for cation transport across plasma membranes. From 35 to 100% of the whole amount of ATP resynthesized via glycolysis is utilized for transport purposes. The experimental results and theoretical calculations suggest that in glucose-containing media Na+ transport increases from 0.75 to 1.78 pmol/hour on a per cell basis. The activation of Na+ transport is due to the exchange of protons formed via glucose conversion into lactate for Na+, i.e., to the stimulation of Na+/H+ antiport. The permeability of plasma membranes for K+ increases 2.75-fold, while the passive flux of Na+ diminishes. It is concluded that the observed increase in the Na+/K+ ratio in ascite tumour cells is connected with their enhanced ability to synthesize lactic acid. Presumably, glycolysis is one of regulatory mechanisms of intracellular ratios of monovalent cations.

• Authors: S D Kaz'min, I M Danko
• Journal: Biokhimiia (Moscow, Russia) (Biokhimiia) Vol. 51 Issue 1 Pg. 95-102 (Jan 1986) ISSN: 0320-9725 [Print] Russia (Federation)
• Vernacular Title: Energeticheskie osobennosti transporta odnovalentnykh kationov v kletkakh astsitnykh opukholeĭ.
• PMID: 3006802 (Publication Type: Journal Article)

Chemical References

• Cations, Monovalent
• Ouabain
• Sodium
• Sodium-Potassium-Exchanging ATPase
• Potassium

Topics

• Animals
• Biological Transport (drug effects)
• Carcinoma, Ehrlich Tumor (metabolism)
• Cations, Monovalent (metabolism)
• Glycolysis (drug effects)
• Kinetics
• Leukemia P388 (metabolism)
• Leukemia, Experimental (metabolism)
• Lymphoma (metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Ouabain (pharmacology)
• Oxygen Consumption (drug effects)
• Potassium (metabolism)
• Sodium (metabolism)
• Sodium-Potassium-Exchanging ATPase (metabolism)