Abstract |
The periodate- thiocarbohydrazide silver proteinate ( PA-TCH-SP) method was used to study the envelopment process in varicella-zoster virus-infected human melanoma cells. Viral envelopment could be seen at two sites, the nuclear membrane and at virus-induced intracytoplasmic vacuoles. Virus-associated glycoconjugates were detected by the PA-TCH-SP method at the plasmalemma and on the inner membrane of the intracytoplasmic vacuoles. Virion envelopes acquired at the nuclear membrane were PA-TCH-SP negative, whereas those acquired at intracytoplasmic vacuoles were PA-TCH-SP positive. All virions found inside these vacuoles contained periodate-reactive envelopes. Release of virions into the extracellular space, where virtually all virions were PA-TCH-SP positive, appeared to be via exocytosis. Thus, the PA-TCH-SP method identifies glycoprotein incorporation at specific cytoplasmic vacuoles distinct from nuclear envelope, endoplasmic reticulum, and Golgi lamellae. These results suggest that envelopment within the cytoplasm is a stage in the assembly of the varicella- zoster virion.
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Authors | E A Montalvo, R T Parmley, C Grose |
Journal | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
(J Histochem Cytochem)
Vol. 34
Issue 2
Pg. 281-4
(Feb 1986)
ISSN: 0022-1554 [Print] United States |
PMID | 3003184
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Glycoproteins
- Viral Proteins
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Topics |
- Glycoproteins
(analysis)
- Herpesvirus 3, Human
(analysis, ultrastructure)
- Histocytochemistry
(methods)
- Viral Proteins
(analysis)
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