The aim of this study was the design and development of a novel de novo drug delivery system for
cancer chemotherapy. For this purpose,
chitosan (CS) functionalized using
phthalic anhydride followed by 4-cyano, 4-[(phenylcarbothioyl) sulfanyl] pentanoic
acid as a chain transfer agent (CTA) to afford CS-CTA macroinitiator. The synthesized CS-CTA macroinitiator was then copolymerized with
methacrylic acid (MAA) monomer using reversible addition-fragmentation chain transfer (RAFT) polymerization technique to produce
chitosan-graft-poly(
methacrylic acid) (CS-g-
PMAA) graft copolymer. Afterward,
graphene oxide (GO) nanosheets were incorporated into the synthesized copolymer through the physical interactions. The CS-g-
PMAA/GO nanocomposite was loaded with
doxorubicin hydrochloride (DOX) as a universal anticancer
drug. The biocompatibility, DOX-loading capacity, and pH dependent drug release behavior of the developed nanocomposite were also investigated. As the experimental results, as well as superior
biological and physicochemical features of CS and GO, we envision that the developed CS-g-
PMAA/GO nanocomposite may be applied as de novo
drug delivery nanosystem for
cancer chemotherapy.