Spared CA1 pyramidal neuron function and hippocampal performance following antisense knockdown of microRNA-134.

Abstract

OBJECTIVE:

Inhibition of microRNA-134 by an oligonucleotide antagomir (ant-134) has been shown to produce powerful antiseizure effects in multiple models of epilepsy. However, to successfully translate the treatment to the clinic, it is important to assess what potential adverse effects it may have on naive brain tissue.

METHODS:

To investigate this, adult male Sprague-Dawley rats were treated with either ant-134 or a scrambled control sequence. Animals were later assessed for spatial navigation, before ex vivo slices were taken to assess the effects of microRNA-134 knockdown on well-defined measures of intrinsic and synaptic properties.

RESULTS:

Hippocampal field potential recordings determined that silencing of microRNA-134 by ant-134 injection was associated with a reduction in epileptiform activity following application of 9 mmol/L K+ . Nevertheless, rats performed normally in the novel object location test. Action potential waveforms and miniature excitatory synaptic currents recorded in CA1 pyramidal neurons were unaffected by ant-134.

SIGNIFICANCE:

These results demonstrate that ant-134 confers a seizure-protective effect without obvious interference with hippocampal neuronal properties or network function. These findings support further development of this novel approach to epilepsy treatment.

• Authors: Gareth Morris, Gary P Brennan, Cristina R Reschke, David C Henshall, Stephanie Schorge
• Journal: Epilepsia (Epilepsia) Vol. 59 Issue 8 Pg. 1518-1526 (08 2018) ISSN: 1528-1167 [Electronic] United States
• PMID: 29978460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 The Authors. Epilepsia published by Wiley Periodicals, Inc. on behalf of International League Against Epilepsy.

Chemical References

• MIRN134 microRNA, rat
• MicroRNAs
• Oligodeoxyribonucleotides, Antisense
• Potassium

Topics

• Action Potentials (drug effects, physiology)
• Animals
• Disease Models, Animal
• Excitatory Postsynaptic Potentials (drug effects)
• Exploratory Behavior (drug effects)
• Hippocampus (cytology, drug effects)
• In Vitro Techniques
• Male
• MicroRNAs (chemistry, genetics, metabolism)
• Neurons (drug effects, physiology)
• Oligodeoxyribonucleotides, Antisense (pharmacology)
• Potassium (pharmacology)
• Rats
• Rats, Sprague-Dawley