Abstract |
The initial adaptive transcriptional response to nitrogen (N) starvation in Escherichia coli involves large-scale alterations to the transcriptome mediated by the transcriptional activator, NtrC. One of these NtrC-activated genes is yeaG, which encodes a conserved bacterial kinase. Although it is known that YeaG is required for optimal survival under sustained N starvation, the molecular basis by which YeaG benefits N starved E. coli remains elusive. By combining transcriptomics with targeted metabolomics analyses, we demonstrate that the methionine biosynthesis pathway becomes transcriptionally dysregulated in ΔyeaG bacteria experiencing sustained N starvation. It appears the ability of MetJ, the master transcriptional repressor of methionine biosynthesis genes, to effectively repress transcription of genes under its control is compromised in ΔyeaG bacteria under sustained N starvation, resulting in transcriptional derepression of MetJ-regulated genes. Although the aberrant biosynthesis does not appear to be a contributing factor for the compromised viability of ΔyeaG bacteria experiencing sustained N starvation, this study identifies YeaG as a novel regulatory factor in E. coli affecting the transcription of methionine biosynthesis genes under sustained N starvation.
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Authors | Amy Switzer, Dimitrios Evangelopoulos, Rita Figueira, Luiz Pedro S de Carvalho, Daniel R Brown, Sivaramesh Wigneshweraraj |
Journal | Microbiology (Reading, England)
(Microbiology (Reading))
Vol. 164
Issue 11
Pg. 1457-1470
(11 2018)
ISSN: 1465-2080 [Electronic] England |
PMID | 29957170
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoproteins
- Escherichia coli Proteins
- MetJ protein, E coli
- PII Nitrogen Regulatory Proteins
- Repressor Proteins
- Transcription Factors
- glnG protein, E coli
- Methionine
- Protein Serine-Threonine Kinases
- YeaG protein, E coli
- Nitrogen
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Topics |
- Apoproteins
(genetics)
- Escherichia coli
(genetics, metabolism)
- Escherichia coli Proteins
(genetics)
- Gene Deletion
- Gene Expression Regulation, Bacterial
(genetics)
- Methionine
(biosynthesis)
- Nitrogen
(metabolism)
- PII Nitrogen Regulatory Proteins
(genetics)
- Protein Serine-Threonine Kinases
(genetics)
- Repressor Proteins
(genetics)
- Transcription Factors
(genetics)
- Transcription, Genetic
(genetics)
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