Genetically
spastic rats were used for studying the effect on muscle tone of beta-carboline-3-carboxylic
acid methylester (
beta-CCM), an inverse
benzodiazepine (BDZ) agonist, and that of
Ro 15-1788 and
CGS 8216, both putative antagonists of pharmacological actions of BDZs. These animals exhibit pathologically
increased muscle tone, which can be recorded and quantified in the electromyogram (EMG) of the gastrocnemius (GS) muscle.
beta-CCM, 2.5 and 3.0 mg/kg i.p., augmented the tonic activity in the EMG of GS muscle in
spastic rats while it did not modify muscle tone at doses of 1.0 and 2.0 mg/kg.
Diazepam, 0.4 mg/kg i.p., and
Ro 15-1788, 5 mg/kg i.p., but not
CGS 8216, 5 mg/kg i.p., antagonised the effect of the
beta-carboline on muscle tone.
Ro 15-1788, at doses of 0.1-5 mg/kg i.p., did not affect muscle tone in the genetically
spastic rats, whilst at doses of 25-200 mg/kg the imidazodiazepine dose dependently increased the tonic activity in the EMG. The action of
Ro 15-1788, 50 mg/kg i.p., was reversed by
diazepam, 0.4 mg/kg i.p., and
beta-CCM, 2 mg/kg i.p., while
CGS 8216, 5 mg/kg i.p., facilitated the effect of
Ro 15-1788 on muscle tone.
CGS 8216 at doses of 5 and 25 mg/kg i.p. was devoid of any effect on muscle tone, whilst at doses of 50 and 200 mg/kg the pyrazoloquinoline increased the tonic activity recorded in the EMG from the GS muscle of the
spastic rats.(ABSTRACT TRUNCATED AT 250 WORDS)