Abstract |
The five alpha genes of herpes simplex virus 1 are the first set of genes to be expressed after infection. Previous studies have shown that alpha genes resident in eukaryotic cells are induced by infection with herpes simplex virus 1 or 2 but not by other herpesviruses and indicate that the alpha trans-inducing factor was a structural component of the virion. This factor induces genes linked to a bona fide promoter and containing at the 5' end a small sequence derived from the promoter-regulatory domains of alpha genes. We report the sequence of a small DNA fragment shown previously to be capable of expressing the alpha trans-inducing factor in transient expression systems. The only gene encoded in its entirety in this fragment is predicted to specify a 479 amino acid protein with a Mr of 53,053. The precise termini of the 1.74-kilobase mRNA specifying this protein were determined in our 5' and 3' S1 nuclease protection studies.
|
Authors | P E Pellett, J L McKnight, F J Jenkins, B Roizman |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 82
Issue 17
Pg. 5870-4
(Sep 1985)
ISSN: 0027-8424 [Print] United States |
PMID | 2994050
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- DNA, Viral
- Transcription Factors
- Viral Proteins
- Thymidine Kinase
|
Topics |
- Amino Acid Sequence
- Base Sequence
- DNA, Viral
(genetics)
- Enzyme Induction
- Genetic Linkage
- Molecular Weight
- Protein Biosynthesis
- Simplexvirus
(genetics)
- Thymidine Kinase
(genetics)
- Transcription Factors
(genetics)
- Transcription, Genetic
- Viral Proteins
(genetics)
|