MiR-30a-5p inhibits osteosarcoma cell proliferation and migration by targeting FOXD1.

Abstract

Despite a number of studies have emphasized the extensive role of microRNA (miRNA) in the development of multiple cancers, the role of miR-30a-5p in the progression of osteosarcoma (OS) and the underlying mechanism are still limited. We detected the expression level of MiR-30a-5p and forkhead box D1 (FOXD1) in Clinical OS specimens and found that miR-30a-5p was significantly decreased while FOXD1 was markedly increased. Dual luciferase assay confirmed that FOXD1 was directly regulated by miR-30a-5p. In vitro assay showed that inhibitior of FOXD1 suppressed cell proliferation, migration and invasion in MG63 and U2OS cells, while overexpression of FOXD1 promoted OS cell proliferation and migration. In vivo assay further showed the inhibition of tumor growth after knockdown of FOXD1. These results suggested that FOXD1 might play key roles in OS development and progression, and was negatively regulated by miR-30a-5p in OS.

• Authors: Jun Tao, Haibo Cong, Hongyan Wang, Daoqiang Zhang, Chuanjie Liu, Hongxia Chu, Qianfeng Qing, Kunzheng Wang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 503 Issue 2 Pg. 1092-1097 (09 05 2018) ISSN: 1090-2104 [Electronic] United States
• PMID: 29936179 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018. Published by Elsevier Inc.

Chemical References

• 3' Untranslated Regions
• FOXD1 protein, human
• Forkhead Transcription Factors
• MIRN30b microRNA, human
• MicroRNAs

Topics

• 3' Untranslated Regions
• Animals
• Bone Neoplasms (genetics, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Forkhead Transcription Factors (genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Mice, Nude
• MicroRNAs (genetics)
• Osteosarcoma (genetics, pathology)