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Chromatographic characterisation of neurotensin-like immunoreactivity in plasma and tissue extracts from hepatoma patients.

Abstract
To determine the nature of the neurotensin-like immunoreactivity produced by some cases of primary liver carcinoma, plasma and tumour neurotensin-like immunoreactivities were characterised in patients with neurotensin-producing hepatoma by chromatographic analysis in conjunction with radioimmunoassay with C- and N-terminally directed antisera. Gel filtration revealed that the majority of neurotensin-like immunoreactivity extracted from the tumour was co-eluted with synthetic neurotensin, whereas a substantial amount of the plasma immunoreactive neurotensin emerged at the void volume. Tumour neurotensin-like immunoreactivity showed a degree of heterogeneity on gel filtration and high pressure liquid chromatography that contrasted with neurotensin-like immunoreactivity extracted from ileal mucosa, suggesting a different post-translational processing of neurotensin within tumour tissue. The ratio of neurotensin 1-8 to neurotensin 1-13 in the patients' plasma suggested that neurotensin was metabolised in a manner similar to that in healthy subjects. The clinical and biological significance of the ectopic production of neurotensin in some hepatomas remains unclear.
AuthorsY C Lee, A J Bacarese-Hamilton, S M Wood, L H Blumgart, S R Bloom
JournalClinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 149 Issue 1 Pg. 29-36 (Jun 30 1985) ISSN: 0009-8981 [Print] Netherlands
PMID2992844 (Publication Type: Journal Article)
Chemical References
  • Hormones, Ectopic
  • Nerve Tissue Proteins
  • Neuropeptides
  • neurotensin-like immunoreactivity
  • Neurotensin
Topics
  • Carcinoma, Hepatocellular (analysis, blood)
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Hormones, Ectopic (isolation & purification)
  • Humans
  • Liver Neoplasms (analysis, blood)
  • Nerve Tissue Proteins (blood, isolation & purification)
  • Neuropeptides
  • Neurotensin (blood, isolation & purification)

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