| Abstract | 6-Hydroxydopamine(6-OHDA), a specific neurotoxin against sympathetic nerve cells, is a drug already used for purging of bone marrow from neuroblastoma cells before autologous bone marrow transplantation. However, we could not detect significant differences in the toxicity of 6-OHDA against neuroblastoma and other tumor cells under the purging conditions clinically used. In contrast, bone marrow stem cells were much more resistant. The unspecific toxic effect of 6-OHDA is caused by H2O2 or H2O2-derived products which are generated by auto-oxidation in the incubation medium before a significant amount of 6-OHDA is taken up by the cells. Withdrawal of oxygen during the incubation period and subsequent incubation with an oxygen containing medium led to a more specific destruction of neuroblastoma cells which can take up 6-OHDA selectively. |
| Authors | G Bruchelt, J Buck, R Girgert, J Treuner, D Niethammer |
| Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 130
Issue 1
Pg. 168-74
(Jul 16 1985)
ISSN: 0006-291X UNITED STATES |
| PMID | 2992460
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Hydroxydopamines
- Superoxides
- Dithionite
- Ascorbic Acid
- Hydrogen Peroxide
- Oxygen
- Catalase
- Superoxide Dismutase
|
| Topics |
- Ascorbic Acid
(toxicity)
- Bone Marrow
(drug effects)
- Catalase
(diagnostic use)
- Cell Survival
(drug effects)
- Cells, Cultured
- Dithionite
(pharmacology)
- Humans
- Hydrogen Peroxide
(toxicity)
- Hydroxydopamines
(toxicity)
- Neuroblastoma
(drug therapy)
- Oxygen
(toxicity)
- Superoxide Dismutase
(diagnostic use)
- Superoxides
(toxicity)
|
