The anti-apoptotic and anti-inflammatory effect of Lactobacillus acidophilus on Shigella sonnei and Vibrio cholerae interaction with intestinal epithelial cells: A comparison between invasive and non-invasive bacteria.

Abstract

The aim of this study was to compare the effect of Lactobacillus acidophilus on the attachment, invasion, and interaction of Shigella sonnei and Vibrio cholerae with Caco-2 epithelial cells. Also, the anti-apoptotic and anti-inflammatory effect of L. acidophilus was investigated on S. sonnei and V. cholerae interaction with Caco-2 cells as the representatives of invasive and non-invasive intestinal bacteria. It was found that pretreatment with L. acidophilus significantly prevented from adherence and internalization of S. sonnei/V. cholerae and reduced the expression of tumour necrosis factor-α and interleukin-8 in host cells. No significant difference was observed in inhibitory effect of Lactobacilli in V. cholerae and S. sonnei attachment, emphasizing on the role of lactobacilli as a physical barrier in inhibiting direct contact with host cell by competitive exclusion, which may affect attachment and subsequent internalization of both invasive and non-invasive pathogenic bacteria in a same scale. The evaluation of early and late apoptosis in Caco-2 cells exposed to V. cholerae/S. sonnei and pretreated by L. acidophilus indicated no remarkable difference in L. acidophilus anti-apoptotic effect on Caco-2 cells against invasive and non-invasive bacterial infection. Moreover, L. acidophilus by itself showed no apoptotic effect on Caco-2 cells. Statistical analysis revealed that L. acidophilus in S. sonnei infected cells was able to reduce pro-inflammatory immune responses (TNF-α, IL-8 and IL-1β) and NO and PGE2 secretion more strongly compared with V. cholerae infected cells. These data showed for the first time that the protective effect of Lactobacilli, as a probiotic bacterium, in interaction suppression was more in invasive bacteria including S. sonnei than in non-invasive V. cholerae.

• Authors: Shabnam Zeighamy Alamdary, Bita Bakhshi, Sara Soudi
• Journal: PloS one (PLoS One) Vol. 13 Issue 6 Pg. e0196941 ( 2018) ISSN: 1932-6203 [Electronic] United States
• PMID: 29874233 (Publication Type: Comparative Study, Journal Article)

Chemical References

• CXCL8 protein, human
• Interleukin-8
• Tumor Necrosis Factor-alpha

Topics

• Caco-2 Cells
• Epithelial Cells (metabolism, microbiology, pathology)
• Humans
• Inflammation (metabolism, microbiology, pathology)
• Interleukin-8 (metabolism)
• Intestinal Mucosa (metabolism)
• Intestines (microbiology, pathology)
• Lactobacillus acidophilus (growth & development)
• Shigella sonnei (growth & development)
• Tumor Necrosis Factor-alpha (metabolism)
• Vibrio cholerae (growth & development)