HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Synergism between recombinant human interferon and nucleoside antiviral agents against herpes simplex virus: examination with an automated microtiter plate assay.

Abstract
An automated, quantitative, cytopathic effect (CPE) inhibition assay with human fibroblasts in 96-well microtiter plates was used to examine the combination of recombinant human interferon-alpha (rIFN-alpha A) and acyclovir, vidarabine, or dihydroxypropoxymethyl guanine against herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) in vitro. Fifty percent CPE (CPE50) end points, calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, represented 1.7 log reduction in viral yield (50-fold or 98% decrease). Using CPE50 end points of drugs alone and in combination, we defined synergism, additivism, or antagonism with an isobologram plot and a combination index equation. The combinations of rIFN-alpha A plus acyclovir and rIFN-alpha A plus dihydroxypropoxymethyl guanine were highly synergistic against both HSV-1 and HSV-2, whereas the combination of rIFN-alpha A plus vidarabine was additive to mildly synergistic. Combinations of antiviral agents synergistic in cell cultures should be pursued with further studies in animal models of human viral disease and potentially in clinical trials.
AuthorsD M Moran, E R Kern, J C Overall Jr
JournalThe Journal of infectious diseases (J Infect Dis) Vol. 151 Issue 6 Pg. 1116-22 (Jun 1985) ISSN: 0022-1899 [Print] UNITED STATES
PMID2987369 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Antiviral Agents
  • DNA, Recombinant
  • Interferon Type I
  • Nucleosides
  • Vidarabine
  • Ganciclovir
  • Acyclovir
Topics
  • Acyclovir (analogs & derivatives, pharmacology)
  • Animals
  • Antiviral Agents
  • Cells, Cultured
  • Cytopathogenic Effect, Viral (drug effects)
  • DNA, Recombinant
  • Drug Synergism
  • Ganciclovir
  • Interferon Type I (pharmacology)
  • Nucleosides (pharmacology)
  • Simplexvirus (drug effects)
  • Vidarabine (pharmacology)
  • Virus Replication (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: