Inhibition of Neuroinflammation by AIBP: Spinal Effects upon Facilitated Pain States.

Abstract

Apolipoprotein A-I binding protein (AIBP) reduces lipid raft abundance by augmenting the removal of excess cholesterol from the plasma membrane. Here, we report that AIBP prevents and reverses processes associated with neuroinflammatory-mediated spinal nociceptive processing. The mechanism involves AIBP binding to Toll-like receptor-4 (TLR4) and increased binding of AIBP to activated microglia, which mediates selective regulation of lipid rafts in inflammatory cells. AIBP-mediated lipid raft reductions downregulate LPS-induced TLR4 dimerization, inflammatory signaling, and expression of cytokines in microglia. In mice, intrathecal injections of AIBP reduce spinal myeloid cell lipid rafts, TLR4 dimerization, neuroinflammation, and glial activation. Intrathecal AIBP reverses established allodynia in mice in which pain states were induced by the chemotherapeutic cisplatin, intraplantar formalin, or intrathecal LPS, all of which are pro-nociceptive interventions known to be regulated by TLR4 signaling. These findings demonstrate a mechanism by which AIBP regulates neuroinflammation and suggest the therapeutic potential of AIBP in treating preexisting pain states.

• Authors: Sarah A Woller, Soo-Ho Choi, Eun Jung An, Hann Low, Dina A Schneider, Roshni Ramachandran, Jungsu Kim, Yun Soo Bae, Dmitri Sviridov, Maripat Corr, Tony L Yaksh, Yury I Miller
• Journal: Cell reports (Cell Rep) Vol. 23 Issue 9 Pg. 2667-2677 (05 29 2018) ISSN: 2211-1247 [Electronic] United States
• PMID: 29847797 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

Chemical References

• Carrier Proteins
• Cytokines
• Lipopolysaccharides
• Toll-Like Receptor 4
• Formaldehyde
• Cholesterol
• Cisplatin

Topics

• Animals
• Carrier Proteins (metabolism)
• Cholesterol (metabolism)
• Cisplatin (adverse effects)
• Cytokines (cerebrospinal fluid)
• Formaldehyde
• Hyperalgesia (chemically induced, complications, pathology, physiopathology)
• Inflammation (cerebrospinal fluid, complications, pathology)
• Lipopolysaccharides
• Membrane Microdomains (metabolism)
• Mice, Inbred C57BL
• Microglia (metabolism)
• Motor Activity
• Myeloid Cells (metabolism)
• Pain (cerebrospinal fluid, complications, pathology, physiopathology)
• Protein Binding
• Protein Multimerization
• Signal Transduction
• Spinal Cord (pathology, physiopathology)
• Toll-Like Receptor 4 (metabolism)