LGI1 tumor tissue expression and serum autoantibodies in patients with primary malignant glioma.

Abstract

OBJECTIVES:

The Leucine-rich glioma inactivated 1 (LGI1) protein is thought to be implicated in malignant progression of glioma tumors, and mutations in the encoding gene, LGI1, cause autosomal dominant lateral temporal epilepsy, a genetic focal epilepsy syndrome. The aim of this study was to investigate the possible involvement of LGI1 in high-grade glioma-associated epilepsy by analyzing its expression in tumor specimens of patients with and without epilepsy and by searching for LGI1 autoantibodies in the sera these patients.

PATIENTS AND METHODS:

We examined tumor tissue samples from 24 patients with high-grade gliomas (12 with and 12 without epilepsy) by immunoblot and detected variable amounts of LGI1 in tumor tissues from 9/24 (37%) patients.

RESULTS:

LGI1 was detected in 7/12 (58%) patients with epilepsy and in 2/12 (16%) patients without epilepsy (p = 0.0894; Fisher's exact test). Moreover, testing blood sera of five patients for antibodies against LGI1 revealed LGI1 autoantibodies in two patients, both suffering from epilepsy and expressing LGI1 in tumor tissue.

CONCLUSION:

Our findings suggest that there may be a preferential expression of LGI1 in high-grade glioma tumors of patients with epilepsy. We also unveil the presence of serum LGI1 autoantibodies in some patients with high-grade gliomas, where they might play an epileptogenic role.

• Authors: Emanuela Dazzo, Elena Pasini, Sandra Furlan, Dario de Biase, Matteo Martinoni, Roberto Michelucci, Carlo Nobile, PERNO Study Group
• Journal: Clinical neurology and neurosurgery (Clin Neurol Neurosurg) Vol. 170 Pg. 27-33 (07 2018) ISSN: 1872-6968 [Electronic] Netherlands
• PMID: 29723732 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Autoantibodies
• Biomarkers, Tumor
• Intracellular Signaling Peptides and Proteins
• LGI1 protein, human
• Proteins

Topics

• Adult
• Aged
• Astrocytoma (blood, diagnosis, genetics)
• Autoantibodies (blood)
• Biomarkers, Tumor (blood, genetics)
• Brain Neoplasms (blood, diagnosis, genetics)
• Epilepsy, Temporal Lobe (blood, diagnosis, genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Glioblastoma (blood, diagnosis, genetics)
• Glioma (blood, diagnosis, genetics)
• HEK293 Cells
• Humans
• Intracellular Signaling Peptides and Proteins
• Male
• Middle Aged
• Proteins (genetics, metabolism)
• Young Adult