Monoclonal antibody-ricin conjugate cytotoxic to cells expressing the common acute lymphoblastic leukemia antigen (CALLA).

Abstract

The monoclonal antibody PHM-6, which is specific for the common acute lymphoblastic leukemia antigen (CALLA), was conjugated to the plant toxin ricin. Binding of the PHM-6-ricin conjugate to cells via the ricin molecule was blocked by the presence of 100 mM lactose. The IC50 (concentration resulting in 50% inhibition) of the PHM-6-ricin conjugate for the CALLA-positive KM-3 cell line was 280-fold greater than for bone marrow stem cells, indicating the potential of this conjugate for immunological purging of autologous remission marrow.

• Authors: J M Pelham, B F Meyer, R P Herrmann, R E Davis, C L Raphael, N Kraft, R C Atkins
• Journal: Pathology (Pathology) Vol. 19 Issue 2 Pg. 124-30 (Apr 1987) ISSN: 0031-3025 [Print] England
• PMID: 2969499 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Differentiation
• Antigens, Neoplasm
• Ricin
• Neprilysin

Topics

• Antibodies, Monoclonal (immunology)
• Antigens, Differentiation (immunology)
• Antigens, Neoplasm (immunology)
• Bone Marrow (drug effects)
• Cell Line
• Cytotoxicity, Immunologic
• Humans
• Indicator Dilution Techniques
• Leukemia, Experimental (pathology)
• Neprilysin
• Ricin (immunology, metabolism, pharmacology)
• Stem Cells (drug effects)