Comparison of the pyrimidine nucleoside phosphorylase activity in human tumors and normal tissues.

Abstract

The novel fluoropyrimidine, 5'-deoxy-5-fluorouridine (5'-dFUDR) has strong tumor-inhibiting effect without severe cytotoxic effects on the normal cells. This pro-drug is metabolized by the enzyme pyrimidine nucleoside phosphorylase (PN'ase) to the active form 5-fluorouracil. Comparative determinations of pyrimidine nucleoside phosphorylase activity in tissue extracts revealed a significantly higher activity in the human gastrointestinal cancer tissues (6.84 +/- 0.70 nmol/mg protein) than in normal tissues from the same organ (2.37 +/- 0.21 nmol/min/mg protein).

• Authors: Y S Choong, S P Lee, P A Alley
• Journal: Experimental pathology (Exp Pathol) Vol. 33 Issue 1 Pg. 23-5 ( 1988) ISSN: 0232-1513 [Print] Germany
• PMID: 2968275 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Prodrugs
• Floxuridine
• Pentosyltransferases
• Pyrimidine Phosphorylases
• Fluorouracil
• doxifluridine

Topics

• Colonic Neoplasms (enzymology)
• Floxuridine (metabolism)
• Fluorouracil (metabolism)
• Humans
• In Vitro Techniques
• Pentosyltransferases (metabolism)
• Prodrugs (metabolism)
• Pyrimidine Phosphorylases
• Spectrophotometry
• Stomach Neoplasms (enzymology)