Starvation or diabetes decreases the content but not the mRNA of 6-phosphofructo-2-kinase in rat liver.

Abstract

In rat liver, the activity of 6-phosphofructo-2-kinase (PFK-2) decreases upon starvation and in diabetes. Cyclic AMP-dependent phosphorylation of the enzyme is not sufficient to account for this decrease. PFK-2 content was therefore measured by immunotitration and relative PFK-2 mRNA levels were determined by hybridization with cDNA probes. The data are compatible with a posttranscriptional mechanism of regulation that involves decreased translational efficiency of PFK-2 mRNA and (or) increased turnover of the PFK-2 protein.

• Authors: K M Crepin, M I Darville, L Hue, G G Rousseau
• Journal: FEBS letters (FEBS Lett) Vol. 227 Issue 2 Pg. 136-40 (Jan 25 1988) ISSN: 0014-5793 [Print] England
• PMID: 2962885 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Phosphofructokinase-1

Topics

• Animals
• Diabetes Mellitus, Experimental (enzymology)
• Kinetics
• Liver (enzymology)
• Male
• Phosphofructokinase-1 (genetics, metabolism)
• RNA, Messenger (genetics)
• Rats
• Rats, Inbred Strains
• Starvation