Intratumoral evaluation of 3D microvasculature and nanoparticle distribution using a gadolinium-dendron modified nano-liposomal contrast agent with magnetic resonance micro-imaging.
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| Abstract |
The enhanced permeability and retention (EPR) effect is variable depending on nanoparticle properties and tumor/vessel conditions. Thus, intratumoral evaluations of the vasculature and nanoparticle distribution are important for predicting the therapeutic efficacy and the intractability of tumors. We aimed to develop a tumor vasculature evaluation method and high-resolution nanoparticle delivery imaging using magnetic resonance (MR) micro-imaging technology with a gadolinium (Gd)-dendron assembled liposomal contrast agent. Using the Gd-liposome and a cryogenic receiving coil, we achieved 50-μm isotropic MR angiography with clear visualization of tumor micro-vessel structure. The Gd-liposome-enhanced MR micro-imaging revealed differences in the vascular structures between Colon26- and SU-DHL6-grafted mice models. The vessel volumes and diameters measured for both tumors were significantly correlated with histological observations. The MR micro-imaging methods facilitate the evaluation of intratumoral vascularization patterns, the quantitative assessment of vascular-properties that alter tumor malignancy, particle retentivity, and the effects of treatment.
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| Authors | Nobuhiro Nitta, Yoichi Takakusagi, Daisuke Kokuryo, Sayaka Shibata, Akihiro Tomita, Tatsuya Higashi, Ichio Aoki, Masafumi Harada |
| Journal | Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 14 Issue 4 Pg. 1315-1324 (06 2018) ISSN: 1549-9642 [Electronic] United States |
| PMID | 29626524 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved. |
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