Isotype specific immunoregulation: role of Fc alpha R+ T cells and IBF alpha in IgA responses.

Abstract

Fc alpha receptor (Fc alpha R)+ T cell hybridomas (Th HA cells) were derived from fusion of Peyer's patch T helper cells (PP Th A cell) which preferentially select sIgA+ B cells and support IgA responses, with the R1.1 T lymphoma cell line. Expression of Fc alpha R on Th HA cells was extensively characterized by several methods, i.e., immunocytoadherence, colloidal gold-immunoelectron microscopy, FACS and ELISA. All four assays clearly demonstrated expression of Fc alpha R on Th HA cells. The use of FACS and ELISA also suggested the expression of Fc alpha R as well as the presence of lower amounts of Fc mu R on these cell lines. The significance of coexpression of Fc alpha R and Fc mu R is currently under extensive investigation in our group. Solubilized membrane fractions derived from Th HA cells were tested for the presence of Fc alpha R on ELISA and for their ability to support in vitro IgA responses. Both Fc alpha R and Fc mu R were detected in cell membrane fractions of Th HA cells. The cell membrane fraction which contained Fc alpha R, supported in vitro IgA anti-SRBC PFC responses when added to PP B cell cultures in the presence of SRBC. This activity was removed by adsorption to an IgA affinity column. On the other hand, the bound fraction contained sFc alpha R and supported IgA anti-SRBC PFC responses in PP B cell cultures, a response pattern that was similar to those obtained with IBF alpha. Taken together, these results suggest that Fc alpha R and IBF alpha are similar molecules and both exhibit functional activity.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: T Kurita, H Kiyono, M L McGhee, J R McGhee
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 216A Pg. 81-7 ( 1987) ISSN: 0065-2598 [Print] United States
• PMID: 2961213 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• Fc(alpha) receptor
• Immunoglobulin A
• Lymphokines
• Prostatic Secretory Proteins
• Receptors, Fc
• beta-microseminoprotein
• immunoglobulin-binding factors

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, CD
• B-Lymphocytes (immunology)
• Cell Membrane (physiology)
• Clone Cells
• Immunoglobulin A (physiology)
• Lymphokines (physiology)
• Mice
• Prostatic Secretory Proteins
• Receptors, Fc (physiology)
• Solubility
• T-Lymphocytes (immunology)
• T-Lymphocytes, Helper-Inducer (immunology)