The study investigated the role of the
metabotropic glutamate receptor subtype 7 (
mGluR7) in
pain signalling in the dorsal striatum of
sham and neuropathic rats. Supraspinal circuitries involved in the dorsal striatum control of
pain were also explored. In the
sham rats, microinjection of N,N'-bis(diphenylmethyl)-1,2-ethanediamine (
AMN082), a selective
mGluR7 positive allosteric modulator, into the dorsal striatum, facilitated
pain, increased the activity of the ON cells and inhibited the activity of the OFF cells in the rostral ventromedial medulla, and decreased
glutamate levels in the dorsal striatum. Conversely,
AMN082 inhibited
pain and the activity of the ON cells while increased the activity of the OFF cells in rats with spared nerve injury (SNI) of the sciatic nerve.
AMN082 also decreased
glutamate levels in the dorsal striatum of SNI rats. The effect of
AMN082 on
mechanical allodynia and
glutamate release was blocked by 6-(2,4-dimethylphenyl)-2-ethyl-6,7-dihydro-4(5H)-benzoxazolone (
ADX71743), a selective
mGluR7 negative allosteric modulator. Moreover, in the
sham rats,
AMN082 increased the activity of total nociceptive convergent neurons in the dorsal reticular nucleus while in the SNI rats, such activity was decreased. The administration of
lidocaine into the subthalamic nucleus abolished the effect of
AMN082 on the total nociceptive convergent neurons in the
sham rats but not in the SNI rats. Thus, the dual effect of
mGluR7 in facilitating or inhibiting
pain responses may be due to the recruitment of different pathways of the basal ganglia, the indirect or direct pathway, in physiological or pathological conditions, respectively.