Abstract |
MicroRNAs have been reported to play an important role in diverse biological processes and progression of various cancers. MicroRNA-29a has been observed to be downregulated in human lung cancer tissues, but the function of microRNA-29a in lung cancer has not been well investigated. In this study, we demonstrated that the expression levels of microRNA-29a were significantly downregulated in 38 pairs of lung cancer tissues when compared to adjacent normal tissues. Overexpression of microRNA-29a inhibited the activity of cell proliferation and colony formation of lung cancer cells, H1299 and A549. Furthermore, microRNA-29a targeted NRAS proto-oncogene in lung cancer cells. In human clinical specimens, NRAS proto-oncogene was highly expressed in human lung cancer tissues compared to normal tissues. More interestingly, microRNA-29a also sensitizes lung cancer cells to cisplatin (CDDP[Please replace "CDDP" with its expansion in the abstract and also provide expansion for the same in its first occurrence in text, if appropriate.]) via its target, NRAS proto-oncogene. Thus, our results in this study demonstrated that microRNA-29a acted as a tumor suppressor microRNA, which indicated potential application of microRNAs for the treatment of human lung cancer in the future.
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Authors | Xin Liu, Xianping Lv, Qiankun Yang, Huifang Jin, Wenpeng Zhou, Qingxia Fan |
Journal | Technology in cancer research & treatment
(Technol Cancer Res Treat)
Vol. 17
Pg. 1533033818758905
(01 01 2018)
ISSN: 1533-0338 [Electronic] United States |
PMID | 29495918
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- MAS1 protein, human
- MIRN29a microRNA, human
- Membrane Proteins
- MicroRNAs
- Proto-Oncogene Mas
- GTP Phosphohydrolases
- NRAS protein, human
- Cisplatin
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Topics |
- Antineoplastic Agents
(pharmacology)
- Cell Line, Tumor
- Cisplatin
(pharmacology)
- Drug Resistance, Neoplasm
(genetics)
- GTP Phosphohydrolases
(biosynthesis, genetics)
- Gene Expression Regulation, Neoplastic
(genetics)
- Genes, Tumor Suppressor
(physiology)
- Humans
- Lung Neoplasms
(genetics, pathology)
- Membrane Proteins
(biosynthesis, genetics)
- MicroRNAs
(genetics, metabolism)
- Proto-Oncogene Mas
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