Twenty adult Sprague-Dawley outbred rats (10 male and 10 female) were fed a nonpurified diet without or containing
dehydroepiandrosterone acetate (
DHEA 6 g/kg diet) for 11 w.
DHEA-treated animals weighed less than the controls after 6 wk and until the end of treatment. However, only the differences between male groups were statistically significant. Food intake of the
DHEA-fed animals was not affected, but resting heat production was elevated for both sexes. Serum
triglyceride levels and activity of hepatic
glucose-6-phosphate dehydrogenase of the experimental groups were lower than controls. Analyses of body composition indicated
DHEA-treated animals had proportionately less body fat and therefore more body water,
protein and ash than controls. In most cases, differences in body composition were due primarily to effects of
DHEA on the female animals. In a second experiment,
DHEA treatment did not alter urinary
ketone levels nor did it enhance
citrate synthase activity in interscapular brown fat, skeletal muscle, heart or liver. Findings suggest that
DHEA acetate treatment affected
body weight, body composition and utilization of dietary energy by both impairing fat synthesis and promoting fat-free tissue deposition and resting heat production. Possible mechanisms by which
DHEA may affect metabolism are discussed.