Abstract |
Laminin is a high molecular weight glycoprotein found in all basement membranes studied to date. Two subunits of laminin, A and B, have been isolated and characterized from a variety of tumor matrices. Recently we have reported the finding, in human placenta, of a new laminin subunit which we termed M. In the present study we report on the presence of laminin M in placentae of other species such as bovine, rat and mouse. In addition, we have examined laminin extracted from mouse EHS-tumor, rat ED-PYS carcinoma and three human carcinoma cell lines. The laminin subunits were detected by the electroimmunoblot technique using antibodies against mouse, rat and human laminin. Laminin M could not be demonstrated either in the two murine tumors, or in the three different human neoplastic cell lines studied. If the absence of laminin M is the consequence of neoplastic transformation, then studies of the metabolism of this subunit may provide new information on neoplastic transformation and invasion, and a useful marker in tumor diagnosis.
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Authors | M Ohno, A Martinez-Hernandez, N Ohno, N A Kefalides |
Journal | Connective tissue research
(Connect Tissue Res)
Vol. 15
Issue 3
Pg. 199-207
( 1986)
ISSN: 0300-8207 [Print] England |
PMID | 2944712
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Basement Membrane
(analysis)
- Cattle
- Cell Line
- Female
- Humans
- Laminin
(analysis)
- Mice
- Molecular Weight
- Neoplasms
(analysis)
- Neoplasms, Experimental
(analysis)
- Placenta
(analysis)
- Pregnancy
- Rats
- Species Specificity
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